Advanced Scientific Research Methods
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Abstract
Abstract
The paper presents the results of the influence of nutrient media, based on extracts from barley, carrot, and red beetroot wastes, used as a sole nutrient source, on the biomass accumulation, biochemical composition, and antioxidant activity of four Saccharomyces cerevisiae strains, 7P, 9P, 10P, and 11P, isolated from local artisanal sourdoughs. Protein content, carbohydrate content, and antioxidant activity (ABTS) were determined using spectrophotometric methods, while biomass was quantified using the gravimetric method. The tested media supported biomass accumulation ranging from 6.5 ± 0.31 to 11.1 ± 0.02 g L⁻¹ d. w., which was significantly (p ≤ 0.05) higher by 52.3 -134% than that obtained in the control medium. The barley-based medium promoted Saccharomyces biomass production, with increased protein and carbohydrate contents, exceeding those of the control by 9.6-34.9% and 9.5-30.0%, respectively. The beetroot-based medium positively influenced the antioxidant status of the yeast biomass. Specifically, the antioxidant activity of strains grown on this medium ranged from 58.89 ± 2.44% to 72.36 ± 2.36% inhibition, significantly higher (p ≤ 0.05) than those observed in the control medium (23.18 ± 2.16 to 25.43 ± 2.85% inhibition). These findings demonstrate that agri-food waste is an efficient and sustainable substrate for cultivating Saccharomyces cerevisiae and producing biomass with enhanced antioxidant activity.
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@article{Chiselia2026Growth,
title = {Growth, Biochemical Profile and Antioxidant Potential of Saccharomyces Yeasts Cultivated in Agro-Food Waste-Derived Media},
author = {Oleg Chiselița and Natalia Chiseliţa and Nadejda Efremova and Elena Tofan and ANA ROZLOVAN and EVELINA ȚÎBÎRNAC and Pavel Magaz},
journal = {Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Food Science and Technology},
year = {2026},
doi = {10.15835/buasvmcn-fst:2026.0043},
url = {https://doi.org/10.15835/buasvmcn-fst:2026.0043}
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