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Comparison of Modified Lake’s Extender for Cryopreservation of Hungarian Giant and Chrabrany Rabbit Sperm

Eva Kissne Varadi, Jakub Vozaf, Jaromír Vašíček, Jozef Berčík and 7 more

Animals | Nov 3, 2026

Abstract

Abstract

Sperm cryopreservation is the most effective method for the long-term preservation of genetic resources. However, rabbit sperm cryopreservation remains challenging. One of the key factors influencing freezing success is the choice of semen diluent; however, no specific diluent has been established for rabbit semen. In this study, we compared the effectiveness of modified Lake’s diluent and evaluated the cryosurvival of semen from the native Hungarian breed and a native Slovak rabbit breed. Both breeds were subjected to the same static freezing procedure. The following parameters were evaluated: motility, viability, plasma membrane integrity, mitochondrial activity, acrosomal status, and intracellular reactive oxygen species. In fresh samples, viability (93.5% vs. 77.4%), total motility (84.6% vs. 67.0%), and progressive motility (76.1% vs. 47.0%) were significantly higher for the Hungarian Giant than in the Chrabrany rabbit samples. Following cryopreservation, viability (56.4% vs. 43.4%), total (39.2% vs. 20.5%) and progressive (20.6% vs. 8.5%) motility, acrosomal status (72.2% vs. 44.9%) and ROS activity (33.8% vs. 24.6%) were higher in samples from the Chrabrany breed. The results suggest that the modified Lake’s diluent may also be suitable for other rabbit breeds and highlight the importance of developing breed-specific cryopreservation protocols.

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Authors

Researchers on this paper

Eva Kissne Varadi

first | National Centre for Biodiversity and Gene Conservation | ORCID 0009-0002-8428-6052

Jakub Vozaf

middle | Slovak University of Agriculture in Nitra | ORCID 0000-0001-6770-0077

Jaromír Vašíček

middle | Slovak University of Agriculture in Nitra | ORCID 0000-0003-4144-8584

Jozef Berčík

middle | Slovak University of Agriculture in Nitra | ORCID 0009-0000-0741-9822

Andrej Baláži

middle | National Agricultural and Food Centre | ORCID 0000-0003-0303-4794

Peter Chrenek

middle | Slovak University of Agriculture in Nitra | ORCID 0000-0002-4188-1540

Árpád Drobnyák

middle | National Centre for Biodiversity and Gene Conservation | ORCID 0000-0002-4264-5689

Zsuzsa Szabó

middle | National Centre for Biodiversity and Gene Conservation

Éva Török

middle | National Centre for Biodiversity and Gene Conservation

Krisztina Liptói

middle | National Centre for Biodiversity and Gene Conservation | ORCID 0000-0002-9083-7570

Barbara Végi

last | National Centre for Biodiversity and Gene Conservation | ORCID 0000-0002-2343-2055

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Citation

BibTeX

@article{Varadi2026Comparison,
  title = {Comparison of Modified Lake’s Extender for Cryopreservation of Hungarian Giant and Chrabrany Rabbit Sperm},
  author = {Eva Kissne Varadi and Jakub Vozaf and Jaromír Vašíček and Jozef Berčík and Andrej Baláži and Peter Chrenek and Árpád Drobnyák and Zsuzsa Szabó and Éva Török and Krisztina Liptói and Barbara Végi},
  journal = {Animals},
  year = {2026},
  doi = {10.3390/ani16213473},
  url = {https://doi.org/10.3390/ani16213473}
}

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