Vitamin C and Antioxidants Research
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A new spectrophotometric method based on the oxidative coupling reaction of Vitamin C with a Thiosemicarbazone reagent in an acidic medium and in the presence of potassium periodate that yields a stable, water-soluble, dark orange-colored complex exhibiting maximum absorbance at a wavelength of 490 nm.
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A new spectrophotometric method has been developed for the determination of Vitamin C in aqueous solutions. The method is based on the oxidative coupling reaction of Vitamin C with a Thiosemicarbazone reagent in an acidic medium and in the presence of potassium periodate. This reaction yields a stable, water-soluble, dark orange-colored complex exhibiting maximum absorbance at a wavelength of 490 nm. The method obeys Beer’s law within a concentration range of (3 to 120 µg/mL), with a molar absorptivity of (4174.24 L·mol⁻¹·cm⁻¹) and a Sandell’s sensitivity index of (0.0421 µg/cm²); the limit of detection (LOD) is (0.0753 µg/mL), and the limit of quantitation (LOQ) is (0.251 µg/mL). The method has been successfully applied to the determination of Vitamin C in pharmaceutical tablets.
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@article{Hashem2026Novel,
title = {A Novel Spectrophotometric Approach for the Determination of Vitamin C Using Thiosemicarbazone and Its Application to Pharmaceutical Preparations},
author = {Omar Adnan Hashem and Hussam Salih Dakhil Mahdi},
journal = {Central Asian Journal of Theoretical and Applied Science},
year = {2026},
doi = {10.51699/cajotas.v7i4.1745},
url = {https://doi.org/10.51699/cajotas.v7i4.1745}
}FAQ
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