Plant Genetic and Mutation Studies Open access Peer reviewed

EMS-induced Development of Early-maturing Mutants in M3 Generation of Barnyard Millet (Echinochloa frumentacea L.)

Neha Gosai, Suman Khanduri, Sweta Mathur, Maneesha Singh

Indian Journal of Agricultural Research | Jul 20, 2026

Abstract

Abstract

Background: Echinochloa frumentacea (Barnyard millet) is a nutritionally important millet crop with limited genetic variability for crop improvement. The present study aimed to induce genetic variability through Ethyl methanesulfonate (EMS) mutagenesis and develop stable early-maturing mutants in the PRJ-1 variety. Methods: Seeds of PRJ-1 were treated with EMS at 0.4%, 0.6%, 0.8% and 1.4% concentrations and evaluated across M1, M2 and M3 generations under a randomized block design with three replications during 2022-2025 at Shri Guru Ram Rai University, Dehradun, Uttarakhand. Growth, germination and yield-related traits were recorded and analysed statistically. Result: EMS treatments induced significant variability among the studied traits. The 0.6% EMS treatment recorded the highest germination (95.33%), root length (22.07 cm), shoot length (72.67 cm) and plant height (79.33 cm), showing significant improvement over the control (p less than 0.05). Higher EMS concentrations (0.8% and 1.4%) adversely affected germination and growth, indicating mutagenic toxicity. A stable early-maturing mutant line was identified in the M3 generation, exhibiting flowering initiation at 40-45 days after sowing (DAS) and physiological maturity at approximately 65 DAS compared with 90-100 days in the control.

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Neha Gosai

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Suman Khanduri

middle

Sweta Mathur

middle

Maneesha Singh

last

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@article{Gosai2026induced,
  title = {EMS-induced Development of Early-maturing Mutants in M3 Generation of Barnyard Millet (Echinochloa frumentacea L.)},
  author = {Neha Gosai and Suman Khanduri and Sweta Mathur and Maneesha Singh},
  journal = {Indian Journal of Agricultural Research},
  year = {2026},
  doi = {10.18805/ijare.a-6583},
  url = {https://doi.org/10.18805/ijare.a-6583}
}

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