Essential Oils and Antimicrobial Activity
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The findings suggest that Thymus vulgaris essential oil represents a cost‐effective natural source of antibacterial and antioxidant agents.
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ABSTRACT This study aimed to evaluate the antibacterial, antibiofilm, and anti‐virulence potential of Thymus vulgaris essential oil (TvEO), along with its antioxidant activity. GC–MS analysis identified 32 constituents, representing 97.83% of the total composition, with thymol as the predominant compound, accounting for more than 70%. The TvEO exhibited notable bactericidal activity against the tested bacterial strains, with minimum inhibitory concentration (MIC) values ranging from 0.0078% to 0.03125% ( v / v ). Staphylococcus aureus ( S . aureus ) showed the highest sensitivity, with the lowest MIC value (0.0078%). Furthermore, TvEO reduced MRSA biofilm formation by more than 90% at the highest tested concentration (1% v / v ) and decreased established biofilms by approximately 60%. Molecular docking analysis indicated that certain components of TvEO may interact with CrtM and SarA proteins, which are involved in S . aureus biofilm formation and virulence factor production. Moreover, TvEO demonstrated significant antioxidant potential according to DPPH and ABTS assays, with IC 50 values of 8,81 ± 0,02 and 2,93 ± 0,08 mg/mL, respectively, comparable to ascorbic acid. Additionally, molecular docking revealed that caryophyllene and α ‐terpinene exhibited the strongest interactions with (PDB ID: 3QFT). These findings suggest that TEO represents a cost‐effective natural source of antibacterial and antioxidant agents.
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@article{Dakhouche2026Phytochemical,
title = {Phytochemical Composition, In Vitro Antioxidant, Antibacterial, and Antibiofilm Activities of Thymus vulgaris Essential Oil From Tiaret, Algeria},
author = {Soumia Dakhouche and Mouna Mnif and Sami Mnif},
journal = {Chemistry & Biodiversity},
year = {2026},
doi = {10.1002/cbdv.71530},
url = {https://doi.org/10.1002/cbdv.71530}
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