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Effect of flaxseed oil in diet on fatty acid composition in the liver of Nile Tilapia (Oreochromis niloticus)

Ana Carolina de Aguiar, Damila Rodrigues de Morais, Leandra P. Santos, Flávia Braidotti Stevanato and 3 more

Archivos Latinoamericanos de Nutrición | Jun 15, 2026 | 29 citations

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It is demonstrated that increasing amounts of LNA in feed may markedly increase the amounts of alpha-linolenic acid, EPA, and DHA in the liver of Nile tilapia.

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This study analyzed the effects of different concentrations of flaxseed oil (FO) on the proximate composition and the contents of alpha-linolenic acid (LNA, 18:3n-3), eicosapentaenoic (EPA, 20:5n-3), and docosahexaenoic (DHA, 22:6n-3) fatty acids in the liver of cultured Nile tilapia (Oreochromis niloticus). During the five-month culture period, tilapias were given diets with incremental concentrations of FO (0.00%; 1.25%; 2.50%; 3.75%, and 5.00%) as a replacement of sunflower oil (control). There was no significant difference in moisture and ash content in the liver between treatments. Protein values ranged from 12.1% (treatment II) to 13.9% (treatment V) and total lipids ranged from 5.6% (treatment V) to 7.2% (treatment II). There was no significant difference between most treatments. Fatty acid methyl esters (FAMEs) were quantitatively analyzed by capillary gas chromatography against a C(23:0), internal standard. Variations in concentrations (in mg g(-1) of total lipids) of fatty acids between treatment I and treatment V ranged from 4.2 to 51.2 (LNA), from 0.2 to 2.3 (EPA), and from 10.6 to 56.2 (DHA), respectively. This experiment demonstrated that increasing amounts of LNA in feed may markedly increase the amounts of LNA, EPA, and DHA in the liver of Nile tilapia.

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Ana Carolina de Aguiar

first | Universidade Estadual de Maringá | ORCID 0000-0002-5063-7259

Damila Rodrigues de Morais

middle | Universidade Estadual de Maringá | ORCID 0000-0002-7021-5299

Leandra P. Santos

middle | Universidade Estadual de Maringá | ORCID 0000-0001-9956-7004

Flávia Braidotti Stevanato

middle | Universidade Estadual de Maringá

Jeane Eliete Laguila Visentainer

middle | Universidade Estadual de Maringá | ORCID 0000-0002-5815-7903

Nilson Evelázio de Souza

middle | Universidade Estadual de Maringá | ORCID 0000-0002-2813-3513

Jesuí Vergilio Visentainer

last | Universidade Estadual de Maringá

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BibTeX

@article{Aguiar2026Effect,
  title = {Effect of flaxseed oil in diet on fatty acid composition in the liver of Nile Tilapia (Oreochromis niloticus)},
  author = {Ana Carolina de Aguiar and Damila Rodrigues de Morais and Leandra P. Santos and Flávia Braidotti Stevanato and Jeane Eliete Laguila Visentainer and Nilson Evelázio de Souza and Jesuí Vergilio Visentainer},
  journal = {Archivos Latinoamericanos de Nutrición},
  year = {2026},
  doi = {10.37527/2007.57.3.009},
  url = {https://doi.org/10.37527/2007.57.3.009}
}

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