Reproductive Physiology in Livestock Open access Peer reviewed

Metabolic, Hormonal and Body Condition Changes in Melatonin-Implanted Dairy Rams and Flock Pregnancy Rate During the Mating Season: A Longitudinal Field Study

Francesca D. Sotgiu, Claudia Caporali, Antonio Spezzigu, Matteo Sini and 6 more

Animals | Jul 15, 2026

Abstract

Abstract

Reproductive activity imposes significant physiological demands on rams. This study evaluated metabolic and hormonal fluctuations in Sarda rams under semi-extensive management conditions, together with flock reproductive outcomes. Fourteen rams were isolated from ewes, subjected to nutritional flushing, and treated with melatonin implants (3 × 18 mg) before joining the flock. From June to December, body condition score (BCS), non-esterified fatty acids (NEFA), urea, triglycerides, cholesterol, testosterone, faecal thyroid hormone metabolites (FTMs), and faecal corticosteroid metabolites (FCMs) were measured every 45 days. Ewes’ pregnancy rates (PRs) and conception dates were determined by ultrasound scanning to estimate flock reproductive performance. BCS declined (p < 0.05) from June (3.11 ± 0.06) to November (2.80 ± 0.06). In November, NEFA, cholesterol and FCMs peaked (p < 0.05), whereas triglycerides and urea reached the lowest levels (p < 0.05). FTMs peaked in June and November (p < 0.05). Testosterone concentrations were three-fold higher in June than the rest of the mating season (p < 0.05), while overall PR was stable. Despite metabolic and endocrine changes in rams, overall flock reproductive performance remained stable. The final stage of mating season may represent a strategic period for targeted management strategies to sustain welfare and long-term performance.

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Authors

Researchers on this paper

Francesca D. Sotgiu

first | University of Sassari | ORCID 0000-0002-2708-3833

Claudia Caporali

middle | University of Sassari | ORCID 0009-0004-7515-1089

Antonio Spezzigu

middle | ATS Sardegna (Italy) | ORCID 0000-0003-3823-5406

Matteo Sini

middle | University of Sassari | ORCID 0009-0006-1793-7889

Chiara C. Costantino

middle | University of Sassari | ORCID 0009-0008-6908-0439

Andrea Mattu

middle | University of Sassari | ORCID 0009-0000-9307-9229

Valeria Pasciu

middle | University of Sassari | ORCID 0000-0001-9900-5976

Christopher Odey

middle | University of Sassari | ORCID 0000-0001-6663-0994

Francesca Mossa

middle | University of Sassari | ORCID 0000-0001-5517-1307

Fiammetta Berlinguer

last | University of Sassari | ORCID 0000-0002-2500-8063

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Citation

BibTeX

@article{Sotgiu2026Metabolic,
  title = {Metabolic, Hormonal and Body Condition Changes in Melatonin-Implanted Dairy Rams and Flock Pregnancy Rate During the Mating Season: A Longitudinal Field Study},
  author = {Francesca D. Sotgiu and Claudia Caporali and Antonio Spezzigu and Matteo Sini and Chiara C. Costantino and Andrea Mattu and Valeria Pasciu and Christopher Odey and Francesca Mossa and Fiammetta Berlinguer},
  journal = {Animals},
  year = {2026},
  doi = {10.3390/ani16142196},
  url = {https://doi.org/10.3390/ani16142196}
}

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