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Comparative evaluation of physicochemical properties, fatty acid profile, anticholinesterase and anti-inflammatory activities of cold pressed chia, linseed, and rapeseed oils

S. Litim, Sabah Boukeria, S. Amokrane, Chawki Bensouıcı and 7 more

Grasas y Aceites | Jul 23, 2026

Abstract

Abstract

Cold-pressed oils from chia (Salvia hispanica L.), flax (Linum usitatissimum L.), and rapeseed (Brassica napus L.) were evaluated for physicochemical properties, fatty acid composition, and in vitro biological activities. Chia oil showed the highest saponification value and the lowest peroxide and acid values, indicating superior oxidative quality. GC-FID analysis revealed high αα-linolenic acid levels in chia and flaxseed oils (63.3 and 56.25%), whereas rapeseed oil was rich in oleic acid (64.35%). Chia oil exhibited the strongest anti-inflammatory activity (IC50=2.46 mg/mL) (IC50 =2.46 mg/mL). Chia and flaxseed oils also showed stronger acetylcholinesterase and butyrylcholinesterase inhibitory effects than rapeseed oil. These findings suggest that αα-linolenic acid-rich oils, particularly chia oil, possess promising nutritional and bioactive potential, while rapeseed oil may offer better oxidative stability and culinary value.

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Researchers on this paper

S. Litim

first | Applied Natural Sciences

Sabah Boukeria

middle | Applied Natural Sciences | ORCID 0000-0001-7550-763X

S. Amokrane

middle | Biotechnology Research Center | ORCID 0000-0003-3325-7390

Chawki Bensouıcı

middle | Biotechnology Research Center | ORCID 0000-0003-4612-4642

Ramazan Erenler

middle | National Student Clearinghouse Research Center | ORCID 0000-0002-0505-3190

S. Bouhayene

middle | University of Skikda | ORCID 0009-0005-1903-5777

Fouzi Boulkenafet

middle | University of Skikda | ORCID 0000-0002-2576-6200

Areej A. Al-Khalaf

middle | Princess Nourah bint Abdulrahman University | ORCID 0000-0001-9278-0822

Mohammad Ahmad Wadaan

middle | King Saud University | ORCID 0000-0002-8330-6990

Simonetta Lambiase

middle | University of Pavia | ORCID 0000-0003-0846-1162

Fahd A. Al‐Mekhlafi

last | King Saud University | ORCID 0000-0002-8190-7901

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Citation

BibTeX

@article{Litim2026Comparative,
  title = {Comparative evaluation of physicochemical properties, fatty acid profile, anticholinesterase and anti-inflammatory activities of cold pressed chia, linseed, and rapeseed oils},
  author = {S. Litim and Sabah Boukeria and S. Amokrane and Chawki Bensouıcı and Ramazan Erenler and S. Bouhayene and Fouzi Boulkenafet and Areej A. Al-Khalaf and Mohammad Ahmad Wadaan and Simonetta Lambiase and Fahd A. Al‐Mekhlafi},
  journal = {Grasas y Aceites},
  year = {2026},
  doi = {10.3989/gya.0969251.2363},
  url = {https://doi.org/10.3989/gya.0969251.2363}
}

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